Professor
Address: School of Chemistry, Bio21 Institute, University of Melbourne, Parkville, VIC 3010 Australia
Room: Bio 21 Inst. 554
Email: rohair@unimelb.edu.au
Teaching responsibilities
Field of expertise Fundamental and Applied Mass Spectrometry
The ion trap as a complete chemical Laboratory:
We make use of two Nobel Prize winning technologies: the quadrupole ion trap and electrospray ionisation (ESI) to examine the fundamental gas phase chemistry of a cornucopia of ionic species. We use multiple
stages of mass spectrometry with collision induced dissociation and ionmolecule reactions to examine gas phase unimolecular and bimolecular reactions in the following areas:
Reactions of organic ions
ESI precharged ions allow us to study key organic reactions such SN2 reactions, nucleophilic aromatic substitution and transacylation reactions [1].
Transition metals - from catalysis to coordination reactions
The unique multi-trapping capabilities allow us to study catalytic cycles (in collaboration with Prof.Wedd) [2], the synthesis and reactivity of organometallics [3] and metal-ligand reactions (in collaboration with Dr. McFadyen) [4] in the gas phase.
Fundamental properties of gas phase ions derived from biomolecules
We are interested in: (i) developing gas phase ion-molecule reactions as probes of biomolecule structure [5]; and (ii) understanding the fragmentation mechanisms of peptide [6] and oligonucleotide ions [7] with a view to improving the analysis of these biologically important molecules via tandem mass spectrometric techniques. New areas of interest include: gas phase chemistry of non-covalent complexes [5,8]; comparing the fragmentation behaviour of radical cations to their even electron counterparts [9].
Applied studies
In collaboration with Prof. Simpson of the Ludwig Institute for Cancer Research, we have diversified into other areas which utilize ESI/MS as an analytical tool, including: (i) solution phase crosslinking of protein complexes to map sites of interaction [10] and (ii) improving the MS analysis of peptides [11].
For further information visit the O'Hair research page
Selected
Publications:
- O´Hair, R.A.J.; Vrkic, A.K., Org. Bio. Chem., 2003, 1, 745.
- Waters, T.; O´Hair, R. A. J.; Wedd, A. G., J. Am. Chem. Soc., 2003, 125, 3384.
- O´Hair, R. A. J., Chem Comm., 2002, 20.
- Styles, M. L.; O´Hair, R. A. J.; McFadyen, W. D.; Tannous, L.; Holmes, R. J.; Gable, R. W., J. Chem. Soc., Dalton Trans. 2000, 93
- O´Hair, R. A. J.; Androutsopoulos, N. K., Org. Lett., 2000, 2, 2567
- O´Hair, R. A. J., J. Mass Spectrom., 2000, 35, 1377
- Vrkic, A. K.; O´Hair, R. A. J.; Foote, S.; Reid, G.E., Int. J. Mass Spectrom., 2000, 194, 145.
- Vrkic, A. K.; O´Hair, R. A. J., J. Am. Soc. Mass Spectrom., 2004, 15, 716.
- Wee, S.; O´Hair, R. A. J.; McFadyen, W. D., Rapid Commun. Mass Spectrom., 2002, 16, 884.
- Taverner, T.; O´Hair, R. A. J.; Simpson, R. J., J. Biol. Chem. 2002, 277, 46487.
- Kapp E.A.; Schütz F.; Reid G.E.; Eddes J.S.; Moritz R.L.; O'Hair R.A.J.; Speed T.P.; Simpson R.J., Anal. Chem., 2003, 75, 6251
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