Time-Resolved TIRF Microscopy In this project, a total internal reflection fluorescence microscope (TIRFM) will be used, with incorporated time resolution electronics, such that the collection of 2D images of the sample surface as a function of time can be obtained. As a proof of concept, this new methodology will be applied to collect time resolved images of fluorescent dye stained cellular material. It is hoped that analysis of the images will reveal distinctly different fluorescent lifetimes for the fluorescent dyes, and that this time resolved TIRFM methodology will prove capable of distinguishing between the microenvironments of fluorophores in the sample, over the area of a two dimensional, heterogeneous surface.  back Surface%20Imaging.htmlAnna%20Mularski.htmlshapeimage_3_link_0
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Contact Michelle Gee School of Chemistry University of Melbourne, VIC 3010 Australia mlgee@unimelb.edu.au Michelle%20Gee.htmlmailto:mlgee@unimelb.edu.aushapeimage_37_link_0shapeimage_37_link_1